The Gram Stain "Bacteria"

Thick smears will non filthiness properly.

2. Air dry the culture smear and tidy sum it over a gentle flame.

3. Add crystallizing empurpled vilification over the culture, and let stand for 10 to 60 seconds. If the smears argon very thin, the sword can be poured by immediately. Pour sour the stain and rinse the excess stain with a stream of water, being careful not to dampen the culture cancelled the slide.

4. Add the iodine resolution to upper side the smear and let stand for 10 to 60 seconds. This reacts with the crystal violet to form a dark shade of blue. under the microscope, cells which are Gram-positive will appear blue-black. Pour off the iodine solution and rinse the smear with running water. evoke off the excess water.

5. Add a few drops of decolorizer (e.g. ethanol), trickling the solution down the slide. Rinse off with water after 5 seconds (or until the solution is colorless as it runs off the smear). If this step is go on too long, it will decolorize some of the Gram-positive bacteria.

6. Counterstain with grassroots fuchsin solut

Characteristics of prokaryotes and eukaryotes. (2004). Retrieved at: http://esg.www.mit.edu.8001

2. bacteria of the Mycobacterium genus, e.g.

Mycobacterium tuberculosis and Mycobacterium leprae (leprosy), and some another(prenominal) species, do not stain by the Gram order even though their cell walls ingest peptidoglycan, because their cell walls contain a great amount of waxy material (The Gram, 2004). This prevents stain from penetrating the cells. Special methods have to be used to stain these cells involving the use of heat to allow stain to penetrate, and once they are cooled, even dilute acid fails to remove the stain, and so these bacteria are known as acid-fast bacteria. Strictly speaking, they are Gram-negative, but not in the way the term is unremarkably used.

History of the gram stain and how it works. (2004). Retrieved

ion for 40 to 60 seconds. This will stain the Gram-negative organisms red, but will not presume the Gram-positive organisms. Wash off the solution with water. Blot with intoxicated paper to remove excess water, or shake off the water and air dry the slide.

4. The major dif
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